منابع مشابه
Activation of Lipoprotein Lipase
Lipoprotein lipase (LPL) from rat heart acetone powders has been reported to depend on the presence of NH4 + , calcium, or other divalent cations for optimal activity. In addition, the enzyme will not hydrolyze an artificial triglyceride emulsion unless it is converted to an active substrate by the addition of very low density lipoproteins, high density lipoproteins (HDL), or certain peptides c...
متن کاملActivation of lipoprotein lipase by lipoprotein fractions of human serum.
Triglycerides in fat emulsions are hydrolyzed by lipoprotein lipase only when they are "activated" by serum lipoproteins. The contribution of different lipoprotein fractions to hydrolysis of triglycerides in soybean oil emulsion was assessed by determining the quantity of lipoprotein fraction required to give half-maximal hydrolysis. Most of the activator property of whole serum from normolipid...
متن کاملChimeras of Hepatic Lipase and Lipoprotein Lipase
Chimeric molecules between human lipoprotein lipase (LPL) and rat hepatic lipase (HL) were used to identify structural elements responsible for functional differences. Based on the close sequence homology with pancreatic lipase, both LPL and HL are believed to have a two-domain structure composed of an aminoterminal (NHz-terminal) domain containing the catalytic Ser-His-Asp triad and a smaller ...
متن کاملImpaired lipoprotein lipase activation by uraemic and post-transplant sera.
1. Lipoprotein lipase was separated from normal human post-heparin plasma by affinity chromatography and assayed with a 14C-labelled triolein emulsion. No enzyme activity was detected unless whole serum was included in the assay as a source of cofactor, apolipoprotein C-II. 2. After a 10 h fast, serum obtained from 46 normal subjects, eight patients with hypertriglyceridaemia but normal renal f...
متن کاملActivation of lipoprotein lipase. Effects of rat serum lipoprotein fractions and heparin.
Guinea pig serum is deficient in high density lipoproteins (HDL); postheparin lipoprotein lipase (LPL) from it hydrolyzes a triglyceride (TG) emulsion very slowly. The rate is markedly increased by the addition of rat serum and even further by rat serum plus heparin. We have studied further the activation of LPL in this system. Rat serum lipoprotein fractions were isolated by ultracentrifugatio...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1970
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.26.5.545